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Adiponectin and leptin secretion are differentially modulated in subcutaneous and visceral adipose tissue by endoplasmic reticulum stress
Author(s) -
Torre-Villalvazo Ivan,
Bunt Ana E,
Torres Nimbe,
Tovar Armando R
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.570.7
Subject(s) - adipose tissue , adipokine , medicine , endocrinology , adiponectin , leptin , pioglitazone , endoplasmic reticulum , chemistry , unfolded protein response , adipose tissue macrophages , adipocyte , perilipin , white adipose tissue , biology , obesity , insulin resistance , type 2 diabetes , diabetes mellitus , biochemistry
Obesity is associated with altered adipokine secretion but the underlying mechanisms responsible for this phenomenon remains unknown. Endoplasmic reticulum stress (ERS) is common alteration in several cell types during obesity. Objective To evaluate whether ERS alters adiponectin and leptin production in subcutaneus (SAT) and viceral (VAT) adipose tissue in mice and in cultured adipocytes. Methods Mice were fed chow diet supplemented with pioglitazone (120mg/kg) for 2 w, in order to stimulate adipokine production and injected with tunicamicyn (2μg/g body weight) for 8 or 24h before euthanasia. For in vitro analysis, primary SAT and VAT adipocytes were cultured with pioglitazone with or without tunicamicyn for 8 or 24h. Results ATF6 and BiP mRNA increased in SAT and VAT of mice injected with tunicamicyn and in cultured adipocytes but at higher magnitude in VAT. Pioglitazone augmented perilipin, SREBP1 and SCD1 mRNA content as expected but interestingly, tunicamicyn reduced this induction. Adiponectin content in adipose tissue and serum was dramatically reduced in mice injected with tunicamicyn and leptin increased. Conclusions Tunicamicyn induces ERS in both adipose depots but at higher magnitude in VAT. ERS impairs the pioglitazone effect on adipose tissue gene expression. ERS reduces adiponectin secretion but increases leptin production in adipose tissue. This work is supported by CONACyT (84757).