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The role of G0/G1 switch gene 2 in mitotic clonal expansion during adipogenesis
Author(s) -
Choi Hyeonjin,
Lee Hye-Min,
Kim Hyo Jung,
Kim Jae-woo
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.570.6
Subject(s) - gene knockdown , adipogenesis , biology , microbiology and biotechnology , adipose tissue , 3t3 l1 , mitosis , regulator , rna interference , gene , endocrinology , genetics , rna
One of the earliest events occurring in adipogenesis is mitotic clonal expansion which is required for differentiation of 3T3‐L1 cells. In this study, we carried out microarray analysis and analyzed genes which might play an important role during this process. Among them, we sorted out candidates, G0/G1 switch gene 2 (G0S2) which is highly expressed in brown and white adipose tissue and is greatly up‐regulated during mouse 3T3‐L1 adipogenesis. When knockdown of endogenous G0S2 inhibits differentiation and decreases the expression of adipocyte specific genes. Moreover, its knockdown induced apoptosis. G0S 2 is a novel putative target gene of PPAR¥ã. PPAR¥ã, which is most highly expressed in adipose tissue, is known as the master regulator of adipogenesis. The absence of PPAR¥ã in 3T3‐L1 fibroblasts by RNA‐mediated interference knockdown impaired adipocyte differentiation same as knockdown of G0S2. And its knockdown caused apoptosis in 3T3‐L1 preadipocytes. These results show that G0S2 is an important role for differentiation of 3T3‐L1 preadipocytes into adipocytes. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2011‐0030711 and 2011‐0015665) and by Korean Research WCU grant (R31‐2008‐000‐10086‐0) from the Korean Ministry of Education, Science, and Technology.