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A naturally‐occurring flavonoid quercetin inhibits autophagy in human rhabdomyosarcoma cell line
Author(s) -
Dokladny Karol,
Moseley Pope
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.543.5
Subject(s) - autophagy , vacuole , microbiology and biotechnology , quercetin , protein kinase b , chemistry , phosphorylation , biology , cytoplasm , biochemistry , apoptosis , antioxidant
Eukaryotes employ an evolutionarily conserved catabolic process of protein degradation called autophagy. It is a dynamic process in which large protein aggregates, entire organelles, and bulk of cytoplasm is sequestered within double membrane vacuoles and delivered for degradation to lysosomes. Quercetin is a naturally occurred flavonoid. Although the antioxidant properties of quercetin have been demonstrated in numerous studies, its effect on autophagy regulation is not known. The purpose of this study was to investigate the effect of quercetin on autophagy regulation in cell culture model (human rhabdomyosarcoma cell line). We found that quercetin (50 μM and 100 μM) inhibited autophagy demonstrated by Western blot analysis of the LC3 lipidation. Autophagy is a highly controlled process and Akt has been shown to negatively regulate autophagy. In our studies, quercetin‐induced down‐regulation of autophagy was associated with an increase in Akt activation demonstrated by increased level of Akt phosphorylation. We conclude that quercetin appears to be a potent autophagy modulator. Further studies are needed to fully determine cellular and molecular mechanisms of quercetin on autophagy regulation.