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Transcriptomic and Epigenomic Characterization of Mouse Models Simulating Features of Post‐Traumatic Stress Disorder
Author(s) -
Muhie Seid,
Hammamieh Rasha,
Miller Stacy-Ann,
Chakraborty Nabarun,
Jett Marti
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.535.12
Subject(s) - dna methylation , epigenomics , biology , chromatin , transcriptome , epigenetics , histone , microbiology and biotechnology , gene , genetics , gene expression
Social defeat mouse models were used to simulate human post‐traumatic stress disorder (PTSD). C57B6 and BALB/c mice exposed to SJL aggressor mice exhibited behaviors accepted as PTSD‐in‐mouse phenotype. Transcripts in hemi‐brain, blood and heart of mice were assayed using Agilent's genome‐wide arrays, and meta‐analyzed with human PTSD data from gene expression omnibus (GEO). DNA methylation changes in hemi‐brains of mice were assessed using Agilent's tiling arrays, and compared with human DNA methylation data from GEO. About 200 transcripts were differentially regulated in both mice and human data. Induced transcripts were involved in axonogenesis, neuroblast proliferation, response to steroid hormone, apoptosis, inflammation, histone modification and nucleotide biosynthesis. Suppressed transcripts were involved in adaptive immunity, dopamine signaling, DNA methylation, synapse organization, matrix adhesion, nerve impulse transmission, T cell commitment and neural crest cell migration. Hypo‐ & hyper‐methylated genes were associated with immune response, neurogenesis, synaptic transmission, anxiety, and chromatin and protein modifications. Integrative analyses of (mice and human) transcriptomic and DNA methylation data were indicative of validity of social defeat mice as human PTSD models in search of biomarkers of PTSD that are accessible in human blood. Funding: Office of Army Surgeon General