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Characterization of the delta12‐Prostaglandin J2‐Coenzyme A interaction and its role in epigenetic modulation of gene expression
Author(s) -
Farwell Sara Lynn Nicole,
Narayan Vivek,
Kodihalli Ravindra,
Prabhu K. Sandeep
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.535.10
Subject(s) - epigenetics , chromatin , chromatin remodeling , psychological repression , gene expression , transcription factor , epigenomics , histone , microbiology and biotechnology , biology , prostaglandin , gene , chemistry , cancer research , genetics , biochemistry , dna methylation
The histone acetyltransferase (HAT) p300 functions to coordinate transcription activation or repression and chromatin remodeling, which plays a pivotal role in embryonic development, tumorgenesis, asthma, chronic obstructive pulmonary disease, viral infections, and learning and memory disorders. Until now, inhibitors of p300 have been investigated with limited success. We have recently described the role of cyclopentenone prostaglandins metabolites as inhibitors of p300 HAT. In the present study, we demonstrate the ability of a stable cellular metabolite of PGJ 2 , Δ 12 ‐ PGJ 2 , to form a covalend adduct with Coenzyme A using LC‐MS/MS. Here we describe the implications of such an adduct on p300 HAT activity and its likely role in cellular gene expression. NIH DK 077152