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Assessments of Chamber Volumes within Perfusion‐Fixed Human Hearts: Direct Measurements versus 3D Volume Reconstructions
Author(s) -
Howard Stephen Andrew,
Goff Ryan P,
Iaizzo Paul A
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.524.2
Subject(s) - biomedical engineering , volume (thermodynamics) , nuclear medicine , medicine , blood volume , materials science , physics , cardiology , quantum mechanics
MRI is a useful tool for determining cardiac blood volumes in patients. Such determinations are derived from approximations based on an assumed shape, calculated by us sing 2 to 10 individual measurements from a given chamber of interest, which makes inherent assumptions of the chamber shape and anatomy. To address the accuracy of the measured blood volumes by direct measurement of chamber volumes and current clinical measurements for obtaining chamber size, we were able to create 3D models of the blood volumes within human hearts and correlate the measurements between the various methods. Human hearts that were deemed not viable for transplantation and were given for research were perfusion fixed in an end‐diastolic shape. These hearts were suspended in agar gel and imaged with 1.5T or 3T MRI scanner. From the scans, we created 3D computer models of the blood volumes in the various chambers to estimate the end‐diastolic volumes. This approach allowed us to obtain very precise chamber size measurements, which could be compared between hypertensive and control hearts. At the same time we can compare this method to direct measurements of the blood volume using displacement and clinical calculations. Using the 3D models allows us to assess the accuracy of the direct measurements and volume calculations of the cardiac blood volume. Funding by NIH training grant 5T32AR007612 and Medtronic professorship funding.