Premium
Cellular Trafficking of Helical Rosette Nanotubes in Dendritic Cells
Author(s) -
House Nicole,
Fenniri Hicham,
Singh Baljit
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.522.5
Subject(s) - endocytic cycle , microbiology and biotechnology , endocytosis , endosome , chemistry , clathrin , antigen presentation , receptor , biology , immune system , intracellular , t cell , biochemistry , immunology
Self‐assembling helical rosette nanotubes (HRNs), composed of cytosine and guanine, have multiple applications. HRN interactions with dendritic cells (DCs), sentinels of the immune system, are key in determining HRN safety and viability. Splenic classical and plasmacytoid DCs were isolated from female C57/BL6 mice using magnetic cell sorting. HRN were conjugated with the RGD (arginine, glycine, aspartic acid) peptide and the fluorophore FITC in a ratio of 1:10μM respectively. Confocal microscopy was used to visualize the endocytic pathways used, HRN intracellular localization and the real‐time uptake process. HRNs used multiple pathways to take residence within clathrin and caveolin‐coated vesicles, early endosomes, and lysosomes. Able to recognize RGD, the integrin αVβ3 is a potential receptor for receptor‐mediated endocytosis. HRNs co‐localized with major histocompatibility complexes indicating possible direct interaction leading to antigen presentation. HRN exposure stimulated DC maturation as shown by dendrite formation and maturation marker (CD40, CD83) expression. Cell viability was confirmed through microscopy and caspase 3 and 9 expression. These data demonstrate that HRN engage αVβ3 to enter DCs through multiple, presumably redundant, pathways, and interact with antigen‐presenting molecules. Grant Funding Source : National Sciences and Engineering Research Council of Canada