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Innate immune signaling plays a crucial role in the pathogenesis of avascular osteonecrosis of the femoral head
Author(s) -
Okazaki Shunichiro,
Nagoya Satoshi,
Tateda Kenji,
Katada Ryuichi,
Mizuo Keisuke,
Watanabe Satoshi,
Yamashita Toshihiko
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.478.6
Subject(s) - pathogenesis , innate immune system , methylprednisolone , medicine , immune system , femoral head , immunology , anatomy
Objectives Pathogenesis of avascular osteonecrosis of the femoral head (ONF) still remains unclear. We hypothesized that innate immune signaling via toll‐like receptor (TLR) play any role in pathogenesis of ONF. We found that administration of ligands of TLR with steroid treatment induces ONF. The present study verified the hypothesis and evaluated effect of steroid in the development of the ONF. Methods Male Wistar rats (n=120) were randomly divided six groups. Each group rats were given saline or ligands of TLR on day 1 and saline or methylprednisolone on day 2. Animals were sacrificed 1, 3, 7 or 14 days after last injection. Histopathological, biochemical analyses were then performed. Results ONF was observed in groups given ligands of TLR and methylprednisolone treatment. Although the activity of IRF7 did not change, the activity of NF‐κB was decreased after methylprednisolone injection. Conclusions The present study shows that the innate immunity signaling pathway via the TLR, especially MyD88 dependent pathway may play a crucial role in pathogenesis of ONF.