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Oxidative Nucleic Acid Modification and Demodification
Author(s) -
He Chuan
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.470.2
Subject(s) - nucleic acid , uracil , alkb , biochemistry , guanine , cytosine , rna , dna , thymine , chemistry , biology , gene , nucleotide , dna repair
Reversible chemical modifications on nucleic acids and proteins determine cell fates. The five bases that comprise nucleic acids „Ÿ adenine, guanine, cytosine, thymine, and uracil „Ÿ can be chemically and enzymatically modified. These chemical events can have significant biological consequences, particularly for gene expression. I will present chemical strategies we have developed to enrich and sequence novel nucleic acid modifications in mammalian genome. Several AlkB family proteins have been identified in the human genome that may mediate nucleic acids oxidation. Some of these proteins play critical roles in obesity/diabetes and various cancers. I will present our recent results that reveal the exact nucleic acid substrates and cellular function of some of these intriguing enzymes. Based on these discoveries we propose a new mode of biological regulation that depends on reversible RNA modification, for which we termed gRNA Epigenetics h.