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Functional imaging of signal propagation in native and engineered myocardium
Author(s) -
Sarvazyan Narine,
Asfour Huda,
Kay Matthew W
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.459.3
Subject(s) - optical mapping , modalities , biomedical engineering , optical imaging , computer science , native tissue , high resolution , medicine , cardiology , tissue engineering , physics , social science , remote sensing , sociology , optics , geology
There are both similarities and differences between imaging of calcium and electrical activity from excised hearts and engineered tissue constructs. We compare the two and consider critical methodological issues that can affect interpretation of optical mapping data from hearts implanted with cardiac tissue constructs. Dual imaging modalities, including mapping of epicardial electrical activity with tissue metabolic state, are also considered. Such modalities are important to reveal differences between metabolic demands of native and surgically implanted engineered myocardium. The mismatch between the two can lead to graft‐induced arrhythmias, especially in settings of intermittent flow. The differences between native and engineered myocardium can become more apparent when they are studied using physiologically relevant conditions, i.e. when cardiac muscle contracts against a mechanical load. This requires performing experiments using a bi‐ventricular working heart preparation, instead of a Langendorff—based setup. Additional post‐processing steps, such as wavelet‐based analysis, can be then used to minimize effects of motion on optical signals. Lastly, we discuss feasibility of shipping engineered cardiac constructs for implantation and/or high‐resolution optical mapping at a different institution (supported by NIH HL095828 Award).