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Muscle breakdown determines Arginine (ARG) availability during hyperdynamic sepsis in the pig
Author(s) -
Ten Have Gabriella A,
Engelen Marielle P,
Wolfe Robert R,
Deutz Nicolaas E
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.43.7
Subject(s) - sepsis , arginine , nitric oxide , medicine , protein catabolism , endocrinology , chemistry , metabolism , phenylalanine , biochemistry , amino acid
RATIONALE Arginine availability plays a key role in systemic ARG to nitric oxide (NO) conversion, but in sepsis it is unknown which of the organs contribute. METHODS Transorgan ARG kinetics was studied in 12 pigs with Pseudomonas aeroginosa induced hyperdynamic sepsis and 8 controls, using primed‐continuous Phenylalanine (13C6), ARG (guanido‐15N2) and Citrulline (CIT) (ureido‐13C‐5,5‐2H2) infusion. 18 h after start of the sepsis induction, whole body protein breakdown (WbPB), WbArg, WbCit, ARG de novo (WbdenovoArg) and nitric oxide (WbNO) production, and transorgan net balances (NB), disposal and production were measured. Enrichment and concentrations were analysed by LC‐MS. Data were reflected as mean (SE) (in nmol/kg bw/min); stats was done by RM‐ANOVA. RESULTS Sepsis increased WbPB, WbArg, WbNO (p<0.05), but did not change WbdenovoArg and WbCit. Only muscle ARG NB changed from net uptake to net release in the PM pigs (115(26) to −127(58); p=0.0046) and was caused by increased ARG production (326(157) to 762(107), p=0.028) and associated with increased muscle protein breakdown. No changes in gut, liver and lung ARG metabolism were observed. CONCLUSION ARG availability during sepsis is maintained only by stimulated muscle protein breakdown.