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Buccal Swab IL‐1ra in Necrotizing Enterocolitis: A Predictive Biomarker
Author(s) -
Torrazza Roberto Murgas,
Li Nan,
Mai Volker,
Chen Sixue,
Chow Marjorie,
Kim Ee-Kyung,
Neu Josef
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.43.5
Subject(s) - necrotizing enterocolitis , biomarker , medicine , gastroenterology , intestinal permeability , buccal swab , immunology , biology , microbiology and biotechnology , biochemistry
Background Necrotizing Enterocolitis (NEC) is a major cause of death and morbidity in very low birth weight (VLBW) infants. IL‐1ra is the competitive receptor antagonist for IL‐1 a cytokine that promotes inflammation and causes an increase in intestinal epithelial tight junction permeability. Hypothesis Buccal sample proteomic analysis will provide biomarker proteins for NEC prediction. Method buccal swab samples one, two and three weeks prior to the development of NEC of 3 NEC and 3 matched controls (n=6) <32 wk gestational age and <1250g were subjected to 2D‐DIGE and LC‐MS/MS analysis. After identification of 37 altered proteins we chose 3 candidate proteins using a broad systems biologic analysis approach. Western blot analysis showed lower IL‐1ra in patients with NEC. Results IL‐1ra was decreased in VLBW infants with NEC compared to controls (p <0.01). Conclusions our results suggest that IL‐1ra could be a predictive biomarker for NEC. Grant Funding Source : NIH RO1 HD 059143