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Evaluation of oxidative stress and energy metabolism in the heart of rats supplemented with different vitamin D doses
Author(s) -
Santos Priscila Portugal,
Rafacho Bruna,
Ardisson Lidiane,
Gonçalves Andrea,
Pires Vanessa,
Minicucci Marcos,
Azevedo Paula,
Campana Alvaro,
Fernandes Ana Angelica,
Zornoff Leonardo,
Paiva Sergio
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.385.8
Subject(s) - medicine , endocrinology , cholecalciferol , oxidative stress , chemistry , vitamin , glutathione peroxidase , lactate dehydrogenase , vitamin d and neurology , vitamin c , dehydrogenase , enzyme , glutathione reductase , superoxide dismutase , biochemistry
The aim was to evaluate of different vitamin D supplementation doses in the heart of normal rats. Methods 86 male rats allocated into 4 groups: control (C, n=21) received standard chow; D3 (n=22), D5 (n=22) and D10 (n=21) received 3000, 5000 and 10000 IU of cholecalciferol/kg chow for 2 months Results Lipid hydroperoxide was higher in D10 compared to other groups (C=143.8±13.9; D3=143.8±13.9; D5=176.3±16.1; D10=179.6±11.8 nmol/g tissue; p<0.001); the activity of antioxidants enzymes: superoxide dismutase was lower in the supplemented groups (C=21.6±4.1; D3=12.9±1.2; D5=13.2±1.7; D10=13.1±1.4 nmol/g protein; p<0,001) and glutathione peroxidase was lower in D3 and D10 compared to the C (C=40.4±6.2; D3=31.5±4.6; D5=35.5±5.2; D10=29.7±3.1 ìmol/g tissue; p<0,001). Activity of the enzymes: â‐hydroxyacyl coenzyme‐A dehydrogenase was lower in D5 and D10 compared to C and D3(C=69.9±3.8; D3=65.8±4.6; D5=44.7±3.3; D10=34.4±1.8 nmol/mg; p<0.001); citrate synthase was lower in D10 compared to other groups (C=39.8±3.2; D3=40.4±2.8; D5=39.7±2.7; D10=34.5±4.0; umol/g tissue; p=0.003) and lactate dehydrogenase (C=220±6.4; D3=209±3.6; D%=199±4.1; D10=256±3.4 nmol/mg; p<0.001) was higher in D10 compared to other groups. Vitamin D treatment showed dose‐dependent effect. Conclusion Normal rats supplemented with vitamin D3 presented higher oxidative stress and changes of cardiac energy metabolism.