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Effects of high‐fat diet on intestinal permeability and inflammation‐associated tumorigenesis
Author(s) -
Kim Min-Young,
Bae Yun-Jung,
Bak Youn-Kyung,
Kim Jin,
Sung Mi-Kyung
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.376.7
Subject(s) - carcinogenesis , occludin , oxidative stress , inflammation , intestinal permeability , endocrinology , medicine , antioxidant , biology , chemistry , immunology , tight junction , biochemistry , cancer
High‐fat diet induces “chronic low‐grade” inflammation and oxidative stress. Intestinal barrier plays an important role and its disruption leads to the increased permeability implicated pathogenic changes in several diseases. The aim of this study was to investigate the effects of high‐fat diet on intestinal permeability and carcinogenesis. APC min+/ − mice were fed with normal diet(ND) or high fat diet(HD), to examine intestinal permeability and tumorigenesis. Although there was no significant difference in body weight, the total tumor number was higher in HD group than that of ND group. Plasma total antioxidant capacity was lower in HD group, while 8‐OHdG level was higher. Protein expressions of ZO‐1, claudin‐1 and occludin were lower in HD group suggesting intestinal permeability was increased. Plasma MPO and IL‐6 levels were higher in HD group than those of ND group. CD14, the only LPS receptor, plasma level and colonic TLR4 mRNA expression were increased in HD group than ND group. These results suggest that high‐fat diet increases oxidative stress and inflammation induced by endotoxemia disrupting intestinal gap junction proteins which increase membrane permeability and accelerating tumorigenesis. [This research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education, Science and Technology (2010‐0000918) and the SRC program(Center for Food&Nutritional Genomics:2011‐0000918) of the National Research Foundation(NRF) of Korea funded by MEST.] Grant Funding Source : Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education,Science and Technology (2010‐ 0000918) and the SRC program(Center for Food&Nutritional Genomics:2011‐0000918) of the National Research Foundation(NRF) of Korea funded by MEST