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Chronic antibiotic administration increases intestinal mass but inhibits functional adaptation in an intestinal failure piglet model
Author(s) -
Barnes Jennifer L.,
Tappenden Kelly A.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.375.3
Subject(s) - jejunum , ileum , duodenum , antibiotics , gastroenterology , medicine , short bowel syndrome , intestinal mucosa , parenteral nutrition , biology , microbiology and biotechnology
Antibiotic therapy is frequently used in pediatric intestinal failure (IF) patients but the effects on intestinal adaptation are unknown. Neonatal piglets underwent an 80% jejunoileal resection (n=39) and received 80% of nutrient needs parenterally and 20% enterally. The antibiotic NAXEL® was administered for 48 hrs or 7 days and piglets were euthanized 7 days after surgery. Variables of adaptation were assessed, analyzed as a randomized complete block design and considered significant at p≤0.05. The objective was to determine the effect of chronic antibiotic administration on intestinal adaptation in a model of neonatal IF. Long‐term antibiotic use increased intestinal weight evidenced by an increase in ileal and colonic mucosa (mg/cm, p=0.01, 0.004 respectively) and total intestinal mass (g, p=0.03). Protein decreased compared to 48 hrs of antibiotics in all segments (μg/mg mucosa, p=0.02 duodenum, p<0.0001 jejunum, ileum and colon) and protein per cell (μg protein/μg DNA) decreased (p=0.02 duodenum, p=0.003 jejunum). Functional adaptation measured by modified Ussing chambers indicated a decrease in active ion (p=0.006 jejunum, 0.03 colon) and total ion transport (p=0.004 jejunum, p=0.05 ileum) after 7 days of antibiotic compared to 48 hrs. These results suggest that chronic antibiotic administration induces an increase in intestinal mass, but hinders functional adaptation in a model of neonatal IF. Funded by NIH Ruth L. Kirschstein Institutional National Research Service Award 5T32 DK59802.