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Preliminary characterization of secreted bioactive compounds from Bifidobacterium longum with modulatory activity towards enterocytic Fasting Induced Adipocyte Factor (FIAF)
Author(s) -
Pham Priscilla,
Cotten Reese,
Kolinek Travis,
Parker Shaniece,
Vattem Dhiraj,
Maitin Vatsala
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.373.6
Subject(s) - bifidobacterium longum , probiotic , bifidobacterium , secretion , adipose tissue , adipocyte , biology , gut flora , microbiology and biotechnology , bacteria , biochemistry , lactobacillus , fermentation , genetics
Gut bacteria have been shown to influence diet‐related obesity, mediated in part via intestinal FIAF, a circulating lipoprotein lipase inhibitor that modulates fat‐storage in the adipose tissue. Probiotic bacteria and probiotic‐derived bioactive compounds with stimulatory activity towards intestinal FIAF may thus serve a protective function against diet‐related obesity. We have previously shown that secreted factors from Bifidobacterium longum (B. longum) , a resident member of the human gut and a probiotic, significantly increased the levels of FIAF secreted from enterocytes in vitro . The objective of this study was a preliminary characterization of the secreted bioactive compounds from B. longum responsible for the observed increases in enterocytic FIAF levels. Our experiments revealed that the modulatory factors were produced by B. longum primarily during exponential phase and caused a concentration‐dependent increase in FIAF‐secretion. Potent increases in FIAF levels were produced by fractions of molecular weight >50kDa isolated from B. longum cell free supernatants. Heat‐treatment did not impact their ability to increase FIAF levels but freeze‐thaw lead to loss of modulatory activity. Trypsin‐ or pepsin‐treatment of cell free supernatants also led to loss of FIAF‐increasing ability, suggesting that the modulatory factors may be secreted B. longum proteins. This research was funded in part by the Texas State University Research Enhancement Program.