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Plasma alpha‐tocopherol transport studied using deuterium‐labeled collard greens
Author(s) -
Traber Maret G,
Leonard Scott W,
Fu Xueyan,
Grusak Michael A,
Booth Sarah
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.365.5
Subject(s) - chemistry , bioavailability , deuterium , absorption (acoustics) , radiochemistry , analytical chemistry (journal) , chromatography , biology , bioinformatics , physics , quantum mechanics , acoustics
To evaluate the bioavailability and plasma transport of deuterium‐labeled (d)‐alpha‐tocopherol (aT) from food, we used collard greens grown in deuterated water as a source of d‐aT. A single serving of 120 g steamed d‐collard greens (1.2 ± 0.1 mg d‐aT) was eaten by each of 20 healthy adults with a standard breakfast; blood samples were obtained up to 72 h. Plasma aT concentrations were measured using LC/MS in negative mode; the ion abundances of d‐aT and unlabeled aT were determined using selected ion monitoring to detect d‐aT isotopomers; predominant ones were m/z 438–442. The (m/z 438–442)/(m/z 434–446) ratio was 0.63 in collard greens, which was used to correct the plasma d‐aT abundance. Peak plasma d‐aT concentrations (250 ± 130 nmol/L; 1.2% ± 0.6% total a‐T) occurred at 9 to 16 h with an estimated absorption of ~36%. The post‐peak exponential disappearance rate was 0.6 ± 0.2 pools/d, equivalent to a half‐life of 29 ± 8 h. This is the first time that aT bioavailability from food with naturally incorporated d‐aT has been measured. Our findings confirm the relatively limited absorption and slow plasma disappearance of physiologic d‐aT doses. Grant Funding Source : USDA No. 58‐1950‐7‐707, USDA No. 58‐6250‐6‐003, NIH DK081761 , NIH DK069341