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Effects of prebiotic inclusion and chain length on intestinal barrier, histomorphology, and mRNA abundance in obese C57BL/6J mice
Author(s) -
Cephas Kimberly D.,
Mangian Heather F.,
Tappenden Kelly A.,
Swanson Kelly S.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.364.1
Subject(s) - inulin , occludin , prebiotic , cecum , ileum , intestinal permeability , chemistry , cellulose , medicine , endocrinology , food science , biochemistry , biology , tight junction
Obesity is linked with increased intestinal permeability, which may contribute to low grade inflammation. The objective of this study was to test the effects of prebiotics on intestinal permeability, morphology and gene expression in an obese mouse model. Obese 18‐week old, C57 mice (n=6) were randomized to high‐fat diets containing 5% cellulose, 10% cellulose, 10% scFOS or 10% inulin and fed for 28 d. Distal ileum, cecum and colon samples were collected for Ussing chamber, histomorphology, and RT‐qPCR analyses. Cecal epithelial resistance was greater (p<0.05) in mice fed inulin vs. mice fed cellulose or scFOS. Distal colon epithelial resistance tended to increase (p=0.08) in mice fed scFOS vs. mice fed cellulose or inulin. Ileal and cecal crypt depth was greater (p<0.05) in mice fed scFOS or inulin vs. mice fed cellulose. Ileal MUC2 expression was greater (p<0.05) in mice fed 10% cellulose vs. mice fed 5% cellulose, inulin or scFOS. Distal colon AMPK and ZO‐1 expression was greater (p<0.05) in mice fed scFOS vs. mice fed cellulose or inulin. Ileal and cecal occludin expression was lower (p<0.05) in mice fed inulin or scFOS vs. mice fed 5% cellulose. Colon occludin expression was lower (p<0.05) in mice fed inulin or scFOS vs. mice fed 10% cellulose. These data suggest prebiotics improve intestinal permeability in the obese and involve tight junction proteins, but the effects vary regionally depending on prebiotic properties. Grant Funding Source : Internal funding