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Relationship between exercise and high fat diet with oxidative stress parameters in the immune system
Author(s) -
Martinez-Carillo Beatriz Elina,
Valdés-Ramos Roxana,
Guadarrama Ana Laura,
Kormanovski Alexandre
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.34.8
Subject(s) - tbars , oxidative stress , medicine , endocrinology , cd19 , chemistry , immune system , cd3 , catalase , lipid peroxidation , immunology , peripheral blood , cd8
It is currently known that there is a strong relation between diet, the immune system and the production of oxidative stress, but the area where they interact is very broad and diverse. The aim of this study was to determine the relation between the intake of a high fat diet and moderate physical exercise on the production of oxidative stress in young Balb/c mice. Animals were divided into control diet (CD) and high fat diet (HFD) (35% above normal) groups, starting at 21 days of age, during 9 weeks they were put to swim for 30 minutes 5 days a week. Peripheral blood was obtained and lymphocytes were purified for CD3+ and CD19+ determination by flow cytometry. Serum was used for analysis of Carbonylated proteins (CP), TBARS and Catalase (CAT). The CD group showed the following data: CD3+ 58.40% (±1.42), CD19+ 26.49% (±2.98); CP 9.66 mg (±1.25), TBARS 26.81 (±7.28), CAT 45.498.66 (±14 145); the HFD group: CD3+ 55.17% (±1.58), CD19+ 19.07% (±3.72); CP 4.31mg (±0.341), TBARS 14.92 (±6.51), CAT 20,419 (±1300). When comparing the groups, T‐lymphocytes and CP showed no differences, whereas B‐lymphocytes (t=3.39, p<0.001), CAT (t=8.64, p<0.001) y TBARS (t=5.96, p<0.015) were significantly decreased in the HFD group. All indicators showed a decrease with fat intake, apparently indicating that exercise is sufficient to overcome the dietary negative effect.