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Effect of a high carbohydrate or a high fat diet and exercise on the oxidant/antioxidant status in liver
Author(s) -
Valdes-Ramos Roxana,
Guadarrama Ana Laura,
Martinez-Carrillo Beatriz Elina,
Kormanovski Alexandre,
Rocio Cruz Hernandez Teresita
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.34.7
Subject(s) - tbars , antioxidant , glutathione peroxidase , catalase , superoxide dismutase , chemistry , oxidative stress , medicine , food science , carbohydrate , endocrinology , lipid peroxidation , biochemistry , biology
Mexico has the largest percentage of obese adults worldwide with a diet high in simple carbohydrates, low in non‐industrialized cereals and tubers, and increased in high fat animal foods. It is necessary to evaluate the oxidant‐antioxidant and immune systems affected by diets. To evaluate the effect of a high carbohydrate (HCD) and a high fat diet (HFD) and 9 weeks of exercise (E) on liver and serum oxidant‐antioxidant status, three‐week old male Balb/c mice were evaluated for protein (carbonyls) and unsaturated fatty acid (TBARS) stress indicators, total antioxidant status (TAS) and glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT) activity in liver homogenates. Carbonyls (8.68±1.25 nmol/mg protein) and TBARS (0.87±0.26 nmol/mg protein) were higher (F=31.096 p<0.001 & F=43.52 p<0.001 respectively) in controls (C) compared to all experimental groups. TAS (F=40.54 p<0.001) was highest in C (3563.37±862.36 nmol/mg protein). All enzymes were decreased. Experimental diets increased the function of the respiratory chain in mitochondria decreasing ROS production and oxidation. The non‐enzymatic portion counteracted diet, exercise and their combination effects. Financed by UAEM and conducted in conformance with FASEB's Statement of Principles for use of animals in research and education. ALGL's graduate studies were funded by CONACyT.