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In‐silico organogenesis: measuring and modelling vertebrate limb development
Author(s) -
Sharpe James
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.337.3
Subject(s) - organogenesis , in silico , vertebrate , morphogenesis , process (computing) , scale (ratio) , developmental biology , systems biology , computational biology , biology , computer science , focus (optics) , bioinformatics , gene , microbiology and biotechnology , genetics , physics , quantum mechanics , optics , operating system
Organogenesis is a dynamical multi‐scale process in which large‐scale features (such as the geometric conformation of the tissue) directly feeds back onto the regulation of molecular‐scale events such as gene regulation. Our attempts to capture this process must therefore focus as much on morphometry as molecular biology. Here I will describe our work to transform a classical model of developmental biology – the developing vertebrate limb bud – into a quantitative model system for systems biology and multiscale modeling. In particular, we have developed: (a) 3D and 4D quantitative data‐capture tools[1], and (b) new multiscale simulation software. This integrated approach is allowing us to combine two of the primary questions of organogenesis: mechanical morphogenesis (the active cellular movements which lead to shaping of the tissue [2,3]), and molecular patterning (the spatial regulatory networks which control gene expression ‐ [3] and unpublished work).