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Muse cells: a novel type of adult human pluripotent stem cells in mesenchymal tissues and their contribution to tissue repair
Author(s) -
Dezawa Mari
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.335.3
Subject(s) - mesenchymal stem cell , induced pluripotent stem cell , microbiology and biotechnology , stem cell , biology , cell type , cell therapy , cell , stem cell marker , cell culture , stem cell transplantation for articular cartilage repair , adult stem cell , endothelial stem cell , embryonic stem cell , in vitro , genetics , gene
We newly found a unique type of stem cells which we named Multilineage differentiating Stress Enduring (Muse) cells (PNAS, 2010). They can be isolated as cells double positive for mesenchymal marker CD105 and human ES cell marker SSEA‐3 from bone marrow, skin, fat tissues and human cultured mesenchymal cells. They express pluripotent stem cell markers, self‐renew and generate cells representative of all three germ layers. Most importantly, they do not show tumorigenic proliferation. When human Muse cells are infused into tail vein of Nog‐mouse suffering from fulminant hepatitis, human Muse cells integrated into mouse liver and differentiated into hepatocyte having an ability to release human albumin into blood stream. Recently, we found that Muse cells are a primary source of iPS cells in human fibroblasts (PNAS, 2011). When human fibroblasts were separated into Muse and non‐Muse cells and transduced with Yamanaka four factors, iPS cells were generated exclusively from Muse cells, but never from non‐Muse cells. Epigenetic alterations were not seen in non‐Muse cells and some of the major pluripotency markers were not expressed for the entire period of generation. Thus, Muse cells will be beneficial for cell‐based therapy and biomedical research.