Premium
Growth factor retention on decellularized rat liver matrices derived from normal and regenerating liver
Author(s) -
Shupe Thomas,
Zimmerman Cynthia,
Petersen Bryon
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.274.10
Subject(s) - decellularization , extracellular matrix , chemistry , matrix (chemical analysis) , glycosaminoglycan , microbiology and biotechnology , liver regeneration , growth factor , progenitor cell , hepatocyte , regeneration (biology) , biochemistry , biology , stem cell , in vitro , chromatography , receptor
We have reported a method for the decellularization of intact rat livers by perfusion with increasing concentrations of detergents. This procedure results in a decellularized organ that maintains an intact capsule and retains an appropriate distribution of extracellular matrix proteins and associated mucopolysaccharides. Immunoassay demonstrated that many growth factors were retained on the decellularized matrix. Several growth factors known to regulate hepatocyte phenotype, including PDGF, IGFBP‐3 and BMP‐7, were found to be increased in matrices from regenerating livers. Equilibration of the decellularized matrix in serum increased the levels of many of these factors. Decellularization by an alternative, enzyme based method resulted in the retention of a different repertoire of growth factors. The liver progenitor cell line, WB344, was able to colonize these matrices and differentiate into mature hepatocytes, but no differentiation was observed on plastic alone. These data indicate that matrices generated from livers in different growth states and by different methods may each be best suited for specific applications.