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MICROBIAL COLONIZATION PATTERNS OF PIGLETS FED BOTH SOW MILK AND FORMULA IS MORE SIMILAR TO THAT OF EXCLUSIVELY SOW‐REARED THAN FORMULA‐FED PIGLETS
Author(s) -
Wang Mei,
Radlowski Emily C.,
Li Min,
Monaco Marcia H.,
Donovan Sharon M.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.268.3
Subject(s) - prebiotic , temperature gradient gel electrophoresis , pyrosequencing , colonization , zoology , biology , inulin , prevotella , food science , infant formula , microbiology and biotechnology , bacteria , biochemistry , 16s ribosomal rna , genetics , gene
By 6 months, ~30% of U.S. babies receive both breastmilk and formula (combined feeding; CF), but little is known about its effect on microbial colonization, particularly in the presence of prebiotics. Newborn piglets were randomized into 5 groups: FF, FP, SR, CF and CP (n=6 per group). Sow‐reared (SR) remained with the sow 24h/day and formula fed (FF) or FF + prebiotic (FP; 4:1 galactooligosaccharide and inulin) were fed a milk replacer. CF and CF + prebiotic (CP) piglets were sow‐reared for 5d and were then rotated between the sow and formula every 12h. On d21, ascending colon contents were collected. Microbiota was assessed by pyrosequencing and denaturing gradient gel electrophoresis (DGGE). Distance‐based redundancy analysis of DGGE and pyrosequencing data separated FF and FP from SR, CF and CP. CP differed from SR by both methods, while CF tended (p=0.06) to differ from SR by DGGE, but not by pyrosequencing. No differences in microbiota between FF and FP or CF and CP were found. Indicator species analysis showed that SR harbored more Resoburia and Actinobacillus , FF/FP had more Weissella and Enterococcus and CF/CP more Megasphaera and Prevotella . Thus, the three feeding paradigms produced distinct microbial colonization patterns; however, CF was more similar to exclusively SR than FF. Adding prebiotics to FP or CP did not result in colonization patterns more similar to that of SR (BMS Freedom to Discover Award). Grant Funding Source : Bristol‐Meyer‐Squibb