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X‐Ray fluorescence as an assay tool for musculoskeletal nutrition animal research
Author(s) -
Branly Rolando M,
Tulp Orien,
Karam David
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.265.4
Subject(s) - in vivo , backscatter (email) , software portability , biomedical engineering , materials science , fluorescence , muscle tissue , nanotechnology , optics , computer science , medicine , physics , biology , anatomy , microbiology and biotechnology , telecommunications , wireless , programming language
XRF techniques are well established for the measurement of the presence of lead and heavy metals in osseous tissue. The advent of compact pyroelectric crystal x‐ray generators and silicon drift detectors presents a new opportunity for nutrition research that studies muscular, vascular and epithelial tissue. We compare the measurable ratios of Fe/K, Fe/Ca, and Fe/Cl in different bovine muscle tissues to establish a baseline feasibility study in preparation for in‐vivo measurements. The low power and portability characteristics of new technology XRF systems make it possible to examine epithelial tissue characteristics of animals in‐vivo. Limited initial calculations and models with backscattered x‐rays utilizing non‐imaging optics indicate promise. Non‐imaging optics are used to increase the effective collecting area of the spectral detection system in the backscatter configuration. We propose the concept that strategically selected ion ratios in muscle and vascular tissues can aid in quantifying “nutritional health” in laboratory animals. We acknowledge institutional support.