Premium
New roles of erythritol identified via transcriptomic profiling
Author(s) -
Berger Alvin,
Cock Peter,
Hartog Gertjan,
Boesten Daniëlle,
Bast Aalt
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.263.3
Subject(s) - chemistry , nitric oxide , signal transduction , biochemistry , transcriptome , microbiology and biotechnology , gene , gene expression , biology , organic chemistry
Erythritol (ERT) is a polyol bulk sweetener with endothelial‐ and oxidant‐protective roles in diabetic rats and human umbilical vein endothelial cells (HUVECs). To unravel mechanism(s) besides radical scavenging, we studied herein transcriptional effects of ERT in HUVECs under high glucose (HG) conditions using Affymetrix microarrays. In response to ERT under HG (HGERT), 521 genes were changed vs HG (p<0.05; 0.94–1.13 fold change). Over‐represented canonical pathways included: TCA cycle/oxidative phosphorylation/electron transport chain; focal adhesion; insulin signaling; and the polyol pathway. Non‐canonical networks included PIK3R1, NFκB, HNF, XBP1, SOS, and RELA. These canonical and non‐canonical pathways are linked to diabetes onset, insulin signaling and production of adhesion molecules/nitric oxide. Many genes were oppositely regulated with HGERT/HG vs HG/NG (normal glucose) demonstrating protective effects of ERT. Notable changed genes were succinate dehydrogenase complex (SDHC‐down‐regulated), linked to energy production; and interferon α‐inducible protein (IFI6‐up‐regulated), linked to immune responses. In addition to its antioxidant activity, ERT influenced expression of various diabetes‐linked genes and pathways. ERT could be a clinically‐relevant nutritional‐ or pharmaceutical ingredient for prevention and treatment of diabetic complications or vascular damage.