Premium
Although Equally Obese, High Fat Diet (HF)‐Fed Ovariectomized (O) Mice Respond with Greater Adipose Tissue (AT) Inflammation Than Sham‐Operated (S) Mice
Author(s) -
Potter Victoria Jeanne Vieira,
Strissel Katherine,
Obin Martin S,
Greenberg Andrew S
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.252.2
Subject(s) - medicine , endocrinology , adipose tissue , steatosis , inflammation , insulin resistance , ovariectomized rat , obesity , chemistry , hormone
How O and S mice differ in response to HF in regards to AT inflammation, insulin resistance (IR), and hepatic steatosis (HS) was investigated. O and S mice (11 wk, n=16) were fed normal chow diet (NC) for 12 wks and then either switched to HF or continued on NC for 14 wks. Mice were compared for total (MRI) and regional (fat pad weight) body fat, IR (HOMA), HS (mgTG), AT immune cell content (FACS) and AT gene expression (qPCR). Data were analyzed using t‐tests and presented as mean±SEM. O started heavier (33.5±0.8 vs. 27.4±0.8 g, p<0.05) with more body fat (34.6±1.5% vs. 17.6±1.9%, p<0.05) but S gained more rapidly on HF resulting in equally obese mice (48.6±1.0 g, 50.5% fat and 48.4±1.2 g, 47.7% fat for S and O on HF, p=0.24 (g), p=0.12 (% fat)). While neither NC group had HS (between groups, p=0.67), HS scores increased with HF (p<0.05 compared to NC) 2.8‐fold for S (136.3±26.4) and 2.6‐fold for O (127.5±22.5 mgTG/g) (between groups, p=0.81). On NC, O had higher HOMA‐IR (17.4±2.8 vs. 5.2±1.6, p<0.05) whereas both HF groups were IR (34.3±7.8 vs. 23.5±2.3 for O and S, p=0.23). HF increased AT T‐cells with O tending to have more than S (616±237 vs. 205±28 CD3+/10K events, p=0.097). O also had greater AT inflammatory gene expression (+2.1‐fold F480, +2.6‐fold CD11c, +7.3‐fold MCP1, +3.8‐fold IL12p40 compared to S, p<0.05). Postmenopausal women may be more sensitive to HF‐induced metabolic disturbances, an effect that is uncoupled from obesity. Grant Funding Source : NIDDK