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Feeding Blueberry Diets Dose‐Dependently Inhibits Bone Resorption in Young Rats
Author(s) -
Zhang Jian,
Lazarenko Oxana P.,
Badger Thomas M.,
Ronis Martin J.,
Chen Jin-Ran
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.244.5
Subject(s) - bone resorption , weanling , endocrinology , rankl , medicine , osteoclast , resorption , tartrate resistant acid phosphatase , acid phosphatase , chemistry , receptor , biology , activator (genetics) , biochemistry , enzyme
Nutritional status is a critical factor that influences bone development. We previously reported that weanling rats fed AIN‐93G semi‐purified diets supplemented with 10% whole blueberry (BB) powder for only two weeks beginning on postnatal day 21 (PND21) significantly promoted bone formation. However, whether there is an effect of BB diet on bone at lower levels of supplementation or if there are additional effects on bone resorption is unknown. The current study examined the effects of three levels of BB diet supplementation (1, 3, and 5%) for 14 days beginning on PND21on osteoclastic bone resorption. Using peripheral quantitative CT scan of tibia, we demonstrated that bone mineral density and content were dose‐dependently increased in BB rats compared to control AIN‐93G fed animals (p < 0.05). Proteins were isolated from long bone of femurs, and Western blot revealed that RANKL (the cytokine receptor activator of nuclear factor‐κB ligand essential for osteoclast formation) protein expression was dose‐dependently decreased (p<0.05). This was accompanied by decreased expression of PPARγ, RANKL and TRAP (Tartrate‐resistant acid phosphatase) mRNA expression (p<0.05). These results indicate significant stimulation of bone growth by dose‐dependent inhibition of bone resorption after BB consumption during early development. Supported in part by ARS CRIS # 6251‐51000‐005‐03S. Grant Funding Source : USDA / ARS CRIS