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Uterine physiological responses and global gene expression in ovariectomized (OVX) rats treated with soy protein isolate (SPI) or 17‐βestradiol
Author(s) -
Ronis Martin J,
Blackburn Michael,
Shankar Kartik,
Gomez-Acevedo Horatio,
Singhal Rohit,
Dunn Shan,
Badeaux Jamie,
Badger Thomas M
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.243.2
Subject(s) - genistein , daidzein , soy protein , ovariectomized rat , endocrinology , isoflavones , medicine , estrogen , biology , uterus , gene expression , gene , food science , biochemistry
Concerns regarding increased endometrial cancer risk have been raised in women who consume soy products as the result of the estrogenicity of phytochemical components such as the isoflavones genistein and daidzein. Female Sprague‐Dawley rats (N = 20/group) were fed AIN‐93G diets with casein or SPI as the protein source from PND30. On PND50, rats were OVX and 10/group infused s.c. with 5 μg/kg/d 17β‐estradiol (E2) to restore physiological levels. On PND64, uteri were collected, weighed and mRNA isolated for massively parallel signature sequencing (RNAseq) analysis of global gene expression profiles. Uterine wet weight was increased (P<0.05) after E2. However, SPI had no effects on uterus weight. E2 significantly regulated expression of 4,344 genes ± >2‐fold. In contrast, SPI feeding changed expression of only 457 genes, of which only 226 overlapped with E2. Many of these genes were regulated in opposite directions by E2 and SPI. 3,129 genes changed in response to E2 + SPI overlapped with E2 alone suggesting suppression of 25% of E2‐regulated genes after SPI consumption. Ki67 mRNA, a marker of cell proliferation demonstrated this pattern of response when examined by real time RT‐PCR. These data suggest SPI feeding does not produce uterine hyperplasia and actually inhibits the proliferative effects of E2 on the uterus. USDA, CRIS 6251‐51000‐007‐03S.