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Bone marrow‐derived multipotent adult progenitor cells are capable of functional sinus node myocytes differentiation
Author(s) -
Zhang Chuansen,
Zhang Xi,
Liu Yanchun
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.209.3
Subject(s) - myocyte , progenitor cell , microbiology and biotechnology , bone marrow , embryonic stem cell , biology , medicine , chemistry , stem cell , immunology , biochemistry , gene
Although recent studies of biological pacemaker are focused on overexpression of bioengineered ion channels and genetically modified stem cells. However, short‐term expression of gene and unkown bio‐safety limit the application of current biological pacemakers. A logical approach to recreate an ideal cellular microenvironment in Petri dishes is to hand the task over to differentiated cells. The aim of the present study is to investigate if multipotent adult progenitor cells(MAPCs) can be induced into pacemaker‐like cells by co‐culture assays. After 1 week co‐culture with neonatal sinus node myocytes, MAPCs from bone marrow of rat expressed HCN2 and HCN4 mRNA and proteins until 4 week. HCN4 mRNA was more abundant than HCN2. The Kir 2.1 and Kir 2.2 that are responsible for inward‐rectifier K + channels were decreased with culture time. The co‐cultured MAPCs showed sinus node myocytes‐like action potentials and drived quiescence cardiomyocytes to beat. These results suggest that MAPCs could differentiate towards functional sinus node myocytes when co‐cultured with neonatal sinus node myocytes.