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Repeat RNAs in Chromosome Regulation and Mis‐Regulation in Cancer
Author(s) -
Lawrence Jeanne Bentley,
Hall Lisa L,
Carone Dawn M
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.203.4
Subject(s) - biology , euchromatin , rna , heterochromatin , genetics , intron , gene , chromosome , long non coding rna , computational biology
This talk will discuss recent findings supporting broad roles of repeat RNAs in chromosome structure and regulation. Earlier we demonstrated that RNA hybridization using the “Cot‐1” (repetitive) human genomic fraction as a probe provides a convenient assay for transcriptional silencing of a chromosome, evident as a region lacking in Cot‐1 RNA signal. While the copious Cot‐1 RNA signal is widely assumed to reflect repeats in introns of expressed genes, our findings indicate otherwise. In dissecting the components of Cot‐1 RNA, we unexpectedly found that particular human satellites are silent in normal cells but grossly over‐expressed nuclear repeat RNAs in cancer. Other findings implicate RNA from particular types of interspersed repeats in the regulation of euchromatic versus heterochromatic chromosomes. Broad implications for the role of “junk” DNA/RNA will be discussed.