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Detection and quantification of 7,12‐dimethylbenzanthracene (DMBA) in the liver and adipose tissue by HPLC
Author(s) -
Jesus Patricia,
Gonçalves Nuno,
Costa Joana,
Pereira Felipe,
Pereira Mariette,
Cabrita Silvério
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.145.19
Subject(s) - dmba , adipose tissue , carcinogen , liver tissue , chemistry , white adipose tissue , cigarette smoke , physiology , liquid paraffin , medicine , endocrinology , cancer , toxicology , biochemistry , biology , carcinogenesis , chemical engineering , engineering
DMBA is an environmental pollutant present in cigarette smoke, automobile exhaust, grilled food, woodsmoke, etc [1] . It is the one of the most potent carcinogenic polycyclic aromatic hydrocarbons, can induce in experimental animals not only skin tumors, but also lung and mammary cancers [2] . The objective of this study is the morphological and molecular evaluation in the experimental administration of 7,12‐dimetilbenzantraceno (DMBA). These evaluations were performed in liver and adipose tissue because the toxic is metabolized in the liver and appear to accumulate in adipose tissue. Ten Wistar male rats, 8 weeks old were submitted to a quarantine period of one week, and then distributed by two groups: the test group, submitted to the oral administration of DMBA 20mg/kg/day, three times a week (Monday, Wednesday and Friday), for four weeks and the control group, kept on the standard conditions. The results of this study, lead us to think that DMBA can become stored on the adipose tissue, with a correlation with the concentration on the liver.