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Evidence for proliferation of airway‐delivered donor type II cells in lungs of recipient mice following intratracheal bleomycin
Author(s) -
Wang Ping M.,
Martin William J
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.143.8
Subject(s) - bleomycin , immunostaining , lung , pathology , surfactant protein c , airway , alveolar cells , alveolar epithelium , medicine , andrology , biology , immunology , immunohistochemistry , epithelium , chemotherapy , surgery
As a model of cell‐based therapy for acute lung injury, we used airway delivery of donor alveolar type II (AT II) epithelial cell into recipient mice after intratracheal instillation of bleomycin (3.33 U/kg). We harvested and purified donor AT II cells from wild type or GFP healthy male adult murine lungs and airway delivered 10^6 cells into female adult recipient mice at day 4 post‐bleomycin. Lungs were isolated and sectioned at day 6 and 18–21 following post bleomycin. We monitored the donor cells pre‐labeled with DiI or with GFP expression in the recipient lungs and identified AT II cells by immunostaining with surfactant protein C (SPC) using microscopy. We found GFP expression in lung tissue to become more diffuse from day 2 to 14 after airway delivery of GFP donor AT II cells. There was co‐expression of ki67, a marker for proliferation, and Dil or GFP labeled AT II cells identified by SPC, indicating the ability of donor AT II cells to proliferate in recipient lungs. Furthermore, we found evidence of adherens junctions (stained with E‐cadherin) between donor AT II cells and recipient alveolar cells labeled with SPC. These results suggest that donor AT II cells following airway delivery proliferate and incorporate within alveolar structures in bleomycin‐injured lungs providing evidence for these cells to act as repair cells during acute lung injury.