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Hyperhomocysteinemia decreases intestinal motility leading to constipation
Author(s) -
Givvimani Srikanth,
Munjal Charu,
Narayanan Nithya,
Tyagi Geetansh,
Metreveli Naira,
Tyagi Suresh
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1163.6
Subject(s) - hyperhomocysteinemia , motility , medicine , pathogenesis , homocysteine , endocrinology , barium meal , constipation , western blot , inflammation , inflammatory bowel disease , chemistry , gastroenterology , biology , biochemistry , disease , genetics , gene
Elevated levels of plasma homocysteine (Hcy) known as hyperhomocysteinemia (HHcy), has been implicated in inflammation and remodeling of intestinal vasculature and it also aggravates the pathogenesis of inflammatory bowel disease (IBD). Interestingly, colon is the pivotal site that regulates Hcy levels in the plasma. We hypothesize that hyperhomocysteinemia decreases intestinal motility through MMP‐9 induced intestinal remodeling leading to constipation. Method To verify this hypothesis we used C57BL/6J or wild type (WT), CBS+/−, MMP‐9 −/− and MMP‐9−/− + Hcy mice. Intestinal motility was assessed by barium meal studies and daily feces output. Expression of ICAM‐1, iNOS, MMP‐9 and TIMPs were studied by western blot and immunohistochemistry. Results Barium meal studies suggest that intestinal motility is significantly decreased in CBS−/+ mice compared to other groups. Fecal output to body weight ratio was significantly reduced in CBS−/+ mice compared to other groups. There was significant up‐regulation of MMP‐9, iNOS, and ICAM‐1 expression in the colon from CBS−/+ mice compared to WT mice. Conclusions Results suggest that HHcy decreases intestinal motility due to MMP‐9 induced intestinal remodeling leading to constipation.