Premium
Changes in innate immune pathway and proteins of Caenorhabditis elegans during Cronobacter sakazakii infection
Author(s) -
BALAMURUGAN KRISHNASWAMY,
Sivamaruthi BhagavathiSundaram
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1156.2
Subject(s) - caenorhabditis elegans , cronobacter sakazakii , biology , pathogen , microbiology and biotechnology , mutant , host (biology) , innate immune system , immune system , bacteria , immunology , gene , genetics
Caenorhabditis elegans is a potent model system for the study of host‐pathogen interactions. C. sakazakii is an opportunistic pathogen that infects and cause mortality in C. elegans . The role of host immune regulatory pathways and proteomic changes in host during C. sakazakii infection was analyzed. Feeding based assay, bacterial colonization, Chemotaxis assay and Pharyngeal pumping assay were performed using MAPK mutant C. elegans . Total protein extracted from the infected host was analyzed by SDS‐PAGE. C. sakazakii infected MAPK mutants ( sek‐1 and pmk‐1) died with the LT 50 of 120 ± 6 h and 96 ± 6 h, respectively. Lawn avoidance behavior was not observed in the mutant worms exposed to C. sakazakii . Bacterial accumulation and Pharyngeal pumping rate was measured. SDS‐PAGE studies revealed the variation in C. elegans total proteins after exposure with C. sakazakii . Comparative analysis of host proteins from previous reports revealed that C‐type lectin and UN coordinated family members were regulated during infection. [This work has been financially supported by CSIR, DST, ICMR and DBT, Government of India, New Delhi, India] .