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Coordinated microRNA/gene networks central to nutrient responsiveness in the 0.9G baboon fetal kidney
Author(s) -
Cox Laura A,
Glenn Jeremy P,
Lange Kenneth P,
Nijland Mark J
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1151.8
Subject(s) - microrna , biology , transcriptome , baboon , gene , gene regulatory network , transcription factor , in silico , genetics , epigenome , regulation of gene expression , gene expression , gene expression profiling , kidney development , computational biology , dna methylation , embryonic stem cell , endocrinology
MicroRNAs are environmentally sensitive gene regulators that play roles in proliferation and differentiation. We hypothesized that miRNAs underlie fetal baboon kidney transcriptome response to nutrient restriction (NR) via coordinated miRNA/gene networks. We used in silico methods to identify NR sensitive miRNAs in fetal kidneys. Differentially expressed genes from 0.9G baboon kidney [CTR, n=3;NR, n=3,70%CTR] were assessed for miRNAs with TargetScan based ID by: genomic location congruency; enrichment analysis; and network analysis to ID hub genes and networks predicted to be regulated by miRNAs. Genomic location congruency identified 145 miRNA families; enrichment analysis ranked 55 families; network analysis revealed 7 families with target sites in 3 or more hub genes. The mir‐27 family has target sites in 7 hub genes and 35 network genes, many transcription factors and all down regulated in NR. Only network analysis provided a directed approach to selecting miRNAs that may coordinately regulate gene expression. Results suggest that miRNAs regulate NR responsiveness in renal development via transcription factors and reveal potential transcriptome/epigenome interactions fundamental to renal development.