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MicroRNA‐126 regulates cardiac angiogenesis induced by aerobic exercise training
Author(s) -
Silva Natan Daniel,
Fernandes Tiago,
Soci Ursula,
Phillips Ian,
Oliveira Edilamar
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1151.5
Subject(s) - angiogenesis , enos , microrna , protein kinase b , medicine , endurance training , pi3k/akt/mtor pathway , gene expression , vascular endothelial growth factor , endocrinology , signal transduction , vegf receptors , biology , gene , microbiology and biotechnology , biochemistry , nitric oxide synthase , nitric oxide
The purpose of this study is investigate the role of microRNA‐126 on cardiac angiogenesis induced by swimming training. Two groups of adult female Wistar rats (n=7/group) received either a sedentary (control), endurance training protocol 1 (T1), or training protocol 2 (T2). GroupT1: TF 60min/day swimming, 5x/week/10week with 5% overload, GroupT2: T1 to same protocol without the 8 th week, 9 th week trained 2x/day, 10 th trained 3x/day. After the training period, hearts were harvested and total RNA isolated. MicroRNA‐126 gene expression analysis was performed by real‐time PCR. We assessed: markers of training, the cardiac capillary/fiber ratio, protein expression of VEGF, Spred‐1, Raf‐1/ERK1/2, PI3K/Akt/eNOS. The cardiac capillary/fiber ratio increased in Trained Groups. VEGF protein expression was increased 42% in T1 and 108 % in T2. Cardiac miRNA‐126 expression increased 26% (T1) and 42% (T2) compared with S. Spred‐1 protein level decreased 41% (T1) and 39% (T2), which consequently increase in angiogenic signaling pathway Raf‐1/ERK1/2. The gene expression of PI3KR2 was reduced 39% (T1) and 78% (T2), which consequently increase in angiogenic signaling pathway PI3K/Akt/eNOS. This study showed the involvement of miRNA‐126 in the regulation of exercise‐induced cardiac angiogenesis, by regulation of its targets that converged in an increase in the VEGF signaling and hence in angiogenic pathways.