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Controlling proteome degradation in Daphnia pulex
Author(s) -
Kemp Catherine Jarrett,
Kültz Dietmar
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1151.15
Subject(s) - daphnia pulex , proteome , proteolysis , pulex , biology , salinity , protein degradation , chemistry , biochemistry , enzyme , zoology , daphnia , ecology , crustacean
Previous two‐dimensional gel electrophoresis (2DGE) studies in Daphnia pulex have described uncontrolled, widespread proteome degradation. An attempted 2DGE study of the D. pulex response to sodium chloride exposure revealed an unexpected result: the previously observed proteolysis is halted following exposure of animals to elevated salinity prior to protein extraction. Further investigation of conditions controlling proteome degradation shows that the proteolytic factor(s) is/are present in an active form in extract from control “normal/healthy” animals; can be negatively regulated by prior in vivo salinity exposure of animals or inhibited by post‐homogenization boiling of samples; and retain proteolytic activity under chemically denaturing conditions. Extended characterization of the environmental and biochemical parameters that influence this degradation are described. Funding provided by NIH grant 5 P42 ES004699‐25, NSF grant IOS‐ IOS‐1049780, UC‐Davis Floyd and Mary Schwall Fellowship in Genetics, UC‐Davis Jastro‐Shields Fellowship.