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Cranberry bioactives augment the activation status of THP‐1 monocytes/macrophages
Author(s) -
Creasy Rebecca A,
Khoo Christina,
Percival Susan S
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.115.7
Subject(s) - thp1 cell line , chemokine , immune system , flow cytometry , secretion , monocyte , chemistry , cranberry juice , tumor necrosis factor alpha , cell culture , microbiology and biotechnology , biology , immunology , biochemistry , endocrinology , genetics , urinary system
Cranberries contain proanthocyanidins (PAC), which have been suggested to interact with immune cell pathogen recognition receptors. We hypothesized that recognition of cranberry PAC would alter cellular function toward an activated state. THP‐1 monocytes were incubated with cranberry fractions and analyzed by flow cytometry with FITC‐CD49a. Cranberry fractions activated monocytes in a time and dose dependent manner as shown by increased expression of CD49a on the surface. Cytokines and chemokines secreted in the culture medium were analyzed by multiplex technology. MIP‐1a, MIP‐1β, and IL‐8 were secreted by the cells without marked secretion of pro‐inflammatory cytokines, IL‐1a, IL‐1β, TNF‐a, and IL‐6. Significantly more MIP‐1a mRNA was present. This suggests compounds found in cranberry can activate monocytes to help regulate immune cell trafficking. A second experiment was designed to determine if cranberry primes immune cells. THP‐1cells were differentiated into macrophages and treated with cranberry fractions or DMSO vehicle for 16 hr. Cells were then washed and challenged with LPS for 6 hr. A trend toward increased MIP‐1a protein was found in cranberry‐treated cells compared to vehicle‐treated cells. Taken together, this suggests that cranberry bioactives prime THP‐1 macrophages for a heightened response upon pathogen challenge. Grant Funding Source : Ocean Spray Cranberries, Inc. and AES, University of Florida