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Effect of Bepridil in postfatigue tension of slow fibers of skeletal muscle
Author(s) -
Vázquez-Rivera Dolores,
Montoya-Pérez Rocío,
Trujillo Xóchitl,
Huerta Miguel,
Sánchez-Duarte Elizabeth,
Cortés-Rojo Christian,
Meza-Carmen Victor,
Saavedra-Molina Alfredo
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1144.13
Subject(s) - bepridil , chemistry , biophysics , electrophysiology , anatomy , tonicity , isometric exercise , biomedical engineering , medicine , calcium , biology , biochemistry , verapamil , organic chemistry
Fatigue is conceived as a reduction in physical strength or a reduction in the maintenance of energy production (Allen et al. 2008). K ATP channels were described in 1983 by Noma; these channels monitored the intracellular levels of ATP and regulate the excitability of the membrane (Kristensen et al. 2006, Tricarico. 2010). Mitochondrial K ATP channels were identified in the mitochondrial inner membrane by Inoue et al. 1991, they play a central role in ischemic pre‐conditioning process, and therefore it is in our interest to study the effect of Bepridil, during post‐fatigue, due to induces mitochondrial swelling and consequently the maintenance of energy production during a period demand of it. Anterior Latissimus Dorsi (ALD) muscles were obtained of chickens, between 1 and 3 weeks old. The muscle was mounted on an experimental recording chamber by placing the proximal end to the bottom of the chamber and the distal end to the hook mechanoelectric transducer (Grass FT03), which through an amplifier and a 320 CyberAmp analog‐digital interface (Digidata 1322A) allowed to acquire by a computer (Pentium 4) the muscle tension generated, date was process with the sofware AXOTAPE (pCLAMP 9.2). Electrical stimulation induced fatigue (pulses of 100 V, 300 ms duration, frequency of 0.2 Hz). The bundle was stimulated until the force decreased by 70 %, then applied Bepridil (1, 10 and 100 μM) for 6 min to observe its effect. We obtained a dose‐response curves of Bepridil, with greater effect at 10 μM over 300 % (p= 0.04 for peak tension and p=0.008 for total tension).