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ROLE OF ESTROGEN THERAPY ASSOCIATED WITH PHYSICAL TRAINING ON OXIDATIVE STRESS PROFILE IN OVARIECTOMIZED RATS
Author(s) -
Brito Sebastião,
Oliveira Brito Janaina,
Bernardes Nathalia,
Dias Danielle,
Llesuy Susana,
Irigoyen Maria-Cláudia,
De Angelis Kátia
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1142.37
Subject(s) - tbars , oxidative stress , ovariectomized rat , endocrinology , medicine , glutathione peroxidase , chemistry , thiobarbituric acid , estrogen , superoxide dismutase , lipid peroxidation
The aim of study was to verify the effects of 17â estradiol therapy (ET) associated or not with physical training (PT) on cardiac, renal and muscular oxidative stress in ovariectomized (OVX) rats. Female Wistar rats were divided into: control (SC), sedentary OVX (SO), sedentary OVX+ET (SOE) and trained OVX+ET (TOE) (n=8 each group). Estradiol pellets were implanted and PT was performed on a treadmill (8wks). Oxidative stress was evaluated by chemiluminescence (CL), thiobarbituric acid reactive substances (TBARS), redox balance of glutathione (GSH/GSSG), catalase (CAT), superoxide dismutase (SOD) and gluthatione peroxidase (GPx) determinations. Oxidative stress was higher in SO (CL, GSH/GSSG: heart and TBARS: kidney and muscle) than in SC group. The SOE and TOE showed reduction compared to SO group in: cardiac CL (SOE:60%; TOE:61%), renal CL (SOE:45%; TOE:55%) and TBARS (SOE:20%; TOE:27%), and muscle TBARS (SOE:84%; TOE:84%). The cardiac GSH/GSSG was higher in SOE and TOE in relation to SO group (SOE: 14.85 ± 2.18; TOE: 16.20 ± 1.76 vs. SO: 4.59 ± 0.82 umol/g). The SOE and TOE showed increased renal CAT, cardiac and renal GPx compared to SO group, attenuating OVX alterations. In conclusion, long‐term ET, associated or not with PT, improved oxidative stress profile in OVX‐rats, suggesting a positive role of these therapies association after menopause. Financial support: 2009/06865‐8; 2007/57595‐5