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Combined Effect of Flow‐Mediated Shear Stress and Resveratrol on Sirt1/PGC‐1 α Pathway in Vascular Endothelial Cells.
Author(s) -
Kim Ji Seok,
Kim Boa,
Park Joon-Young
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1142.21
Subject(s) - resveratrol , vasoprotective , chemistry , mitochondrial biogenesis , shear stress , microbiology and biotechnology , mitochondrion , biophysics , biology , biochemistry , materials science , nitric oxide , organic chemistry , composite material
The concept of enhancing structural integrity of mitochondria has emerged as a novel protective mechanism against CVD. Flow‐mediated laminar shear stress (LSS) and resveratrol (RSV) have vasoprotective effects, in part, by promoting mitochondrial biogenesis through Sirt1/PGC‐1α pathway. Objective The purpose of this study was to test a hypothesis that combined treatment of the two effective stimulators would have synergistic effect on the activation of Sirt1/PGC‐1α pathway in endothelial cells (ECs). Methods HUVECs were exposed to either high LSS ( h ‐flow, exercise‐mimic, 20 dyne/cm 2 ), RSV (20 μM), or h ‐flow + RSV conditions for 12 h. For the h ‐flow conditions, cells were further exposed to low LSS ( l ‐flow, postexercise recovery‐mimic, 5 dyne/cm 2 ) for 24 h. Results RSV treatment increased Sirt1 and PGC‐1α expression levels in a dose‐and time‐dependent fashion. We observed that the Sirt1 protein expression level was further increased under h‐ flow + RSV condition (P < .05). Moreover, h‐ flow resulted in 10‐fold increase in PGC‐1α expression level during l ‐flow (P < .05). However, there was no additive effect of RSV on PGC‐1α expression level at any shear conditions. Conclusion The current preliminary data suggests that, although the combined RSV and h ‐flow treatment has a synergistic effect on Sirt1 expression, h ‐flow may override the effect of RSV with respect to the mitochondrial biogenesis in ECs.