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Modulation of the ubiquitin proteasome system (UPS) in thyroid hormone‐induced cardiac hypertrophy
Author(s) -
LINO CAROLINE ANTUNES,
BARRETO-CHAVES MARIA LUIZA MORAES
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1139.6
Subject(s) - endocrinology , ubiquitin , medicine , thyroid , proteasome , hormone , muscle hypertrophy , blot , radioimmunoassay , heart failure , cardiac hypertrophy , chemistry , gene , biochemistry
The aim of this study is to evaluate the role of the ubiquitin proteasome system (UPS) in thyroid hormone (TH)‐induced cardiac hypertrophy. Experimental hyperthyroidism was induced in male Wistar rats by daily intraperitoneal injections of T3 (7 μg/100 g BW/day) for 21 days. The catalytic subunits of the proteasome were evaluated by real time RT‐PCR and the ubiquitinated proteins were analyzed by Western Blotting. The hyperthyroid status was confirmed by serum TH levels dosage by radioimmunoassay. As expected, the heart rate was increased in hyperthyroid animals from 7th day of treatment (n=3; p<0.001) as well as the cardiac mass, which increased about 31% compared to control (n=3; p<0.001). Although additional experiments need to be performed, the results indicate that there was an increase on gene expression of β1, β2 and β5 subunits of proteasome in hyperthyroid group, while the expression of ubiquitinated proteins remained unchanged when compared to control group. It remains to be clarified how this proteolytic system is modulated in hyperthyroidism condition and if this modulation could contribute to the development of TH‐induced cardiac hypertrophy. Source of Research Support: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP).