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Intermittent dobutamine administration mimicked exercise‐induced cardiac phenotype and protected against left ventricular acute pressure overload
Author(s) -
Gonçalves Daniel,
Henriques-Coelho Tiago,
Fonseca Hélder,
Ferreira Rita,
Padrão Ana Isabel,
Santa Cátia,
Vieira Sara,
Amado Francisco,
Leite-Moreira Adelino,
Duarte José Alberto
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1139.11
Subject(s) - pressure overload , medicine , dobutamine , cardiology , cardiac function curve , muscle hypertrophy , hemodynamics , cardiac hypertrophy , heart failure
The aim of the present study was to address if the submission to a stimuli that mimicked the intermittent cardiac overload imposed by exercise training, would result in a comparable cardioprotective phenotype. Male Wistar rats were submitted to treadmill running (Ex,n=20), dobutamine (Dob; 2mg/kg,s.c.,n=20) or placebo administration (Cont,n=20) for 5 days/week during 8 weeks. After that, half were sacrificed for histological and biochemical analysis and half were submitted to hemodynamic evaluation at baseline and during 2 hours of left ventricular acute pressure (LVP) overload (35% increase in LVPmax). Both Ex and Dob showed cardiac hypertrophy with normal MHC isoforms, fibrosis, osteopontin‐1 and calcineurin protein levels. Moreover, they presented increased SERCA2a and phospho Akt/mTOR protein levels. Both Ex and Dob protected from cardiac dysfunction induced by acute LVP overload. These data suggest that chronic submission to intermittent cardiac overload induced by dobutamine provides a cardioprotective phenotype resembling exercise training.