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Oxidative stress contributes to the cigarette smoke extract induced adverse functional effects of rat cardiac stem cells
Author(s) -
Sumanasekera Wasana K.,
Tran David,
Rokosh Gregg,
Sumanasekera Thimira,
Tran Hieu T.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1137.13
Subject(s) - oxidative stress , reactive oxygen species , context (archaeology) , stem cell , oxidative phosphorylation , superoxide , apoptosis , mitochondrion , vitamin c , chemistry , viability assay , pharmacology , medicine , microbiology and biotechnology , biology , biochemistry , paleontology , enzyme
Cigarette smoke (CS) is a causative agent in several cardiovascular diseases and premature death. CS is composed of many constituents that can modulate cardiac cell functions. The integrity of cardiac cell functions is crucial in maintaining proper cardiovascular health. The main objective of our study is to investigate whether cigarette smoke extracts (CSE) cause impairment of cardiac stem cell functions via oxidative damage. We hypothesized that CSE, via oxidative modifications of proteins and generation of reactive oxygen species can modulate rat cardiac stem cell (RCSC) functions. This is a novel idea and there are few reports discussing CSE induced impairment of endothelial progenitor cell functions via oxidative stress, but not in the context of cardiac stem cells. In our study c‐kit positive RCSC were treated with cigarette smoke extracts (CSE) for 24 hours, and modulations of RCSC functions were assayed. CSE attenuated RCSC viability, migration, and accelerated apoptosis in a dose dependent manner. Anti‐oxidant vitamin C has prevented CSE induced effects. To find out whether oxidative stress play a role in CSE mediated adverse effects, oxidative modification of proteins due to CSE was detected using Oxiblot ™ assay. Compared to control, in CSE treated cells carbonyl groups of proteins were oxidatively modified. The possibility of vitamin C mediated prevention of CSE induced oxidative modification of proteins is currently under investigation. CSE induced generation of reactive oxygen species will be detected using cytochrome C superoxide assay. Research support was provided by Sullivan University College of Pharmacy faculty development grant awarded to Dr. Wasana Sumanasekera.