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Role of NO in the Organization of Ciliated Cells in the Lateral Brain Ventricle
Author(s) -
Fowajuh Ann-Desdemonia Neh,
Peunova Natalia,
Enikolopov Grigori
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1131.4
Subject(s) - ciliogenesis , ciliopathies , cilium , motile cilium , lateral ventricles , biology , ciliopathy , microbiology and biotechnology , ependyma , neuroscience , anatomy , chemistry , central nervous system , genetics , gene , phenotype
Cilia beating in brain ventricles, lungs, trachea and oviduct are important for normal physiology of these organs. Ciliopathies are diseases associated with the dysfunction of ciliogenesis and cilia function. Low nasal nitric oxide (NO) levels are characteristic of ciliopathies; however, the link of NO with ciliogenesis is unknown. The rationale behind the project was to find the link between medical observations of low NO levels in ciliopathies and molecular mechanisms of NO action in ciliogenesis. We hypothesize that NO is critical to ciliogenesis and function and low levels of NO results in dysfunction in the mucociliary tissue in the brain ventricles. Using immunochemistry, the integrity of the pattern of ciliated cells and the polarity of the cytoskeleton in neuronal NO synthase knock out (nNOS KO) and wild type (WT) mice were assessed. The role of NO in cilia function was investigated by recording the movement of fluorescent beads; in the brain ventricles of WT and nNOS KO mice using Imaris Software. Pinwheel structures were found at the ventricular surfaces in neurogenic areas of the adult brain in both WT and KO. However, ciliated cells surrounding neuro stem cells had a higher planar polarity in WT versus nNOS KO mice. Results also show that NO controls cilia planar polarity in the lateral ventricle of mice and a deficiency of NO causes a decrease in cilia driven flow