Premium
The endothelial cells of ductus arteriosus have a unique gene profile to control vascular morphology
Author(s) -
Liu Norika,
Yokota Tomohiro,
Maekawa Shun,
Yokoyama Utako,
Kato Takashi,
Minamisawa Susumu
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1129.6
Subject(s) - ductus arteriosus , morphogenesis , biology , microbiology and biotechnology , gene , fetus , microarray , medicine , gene expression , genetics , pregnancy
Patency of ductus arteriosus (PDA) is morbid; especially it is a severe problem in preterm infants. Hence, to investigate the mechanism of DA closure is important for pediatrics. The present study focused on endothelial cells (ECs) in the DA because ECs lining vessels have a variety of functions and play a central role in homeostasis of the circulatory system. We hypothesized that ECs of the DA had unique characteristics from other vessels and that they should be the primary target to investigate the molecular mechanisms of the DA‐specific vasoconstriction and vascular remodeling. Using fluorescence activated cell sorter, we isolated pure ECs from pooled tissues from the DA or the aorta of Wistar rat fetuses on the 21st day of gestation (e21) or 30 minutes after birth (day0). The gene profiling of those sorted cells were then analyzed by DNA microarray (GeneChip Rat Gene 1.0 ST Array, Affymetrix). We found 61 genes in e21 and 63 genes in day0 were specifically expressed in DA ECs than in aortic ECs (the data were cut off by p value<0.01 or fold change > 2.0). Among them, the genes related to cell adhesion and blood vessel morphogenesis development, such as Vegf‐a, Tgf‐beta, Tbx1, Fgf10 and Pitx2 were abundant in DA ECs, which indicates those genes play an important role in specification of DA morphology and function.