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Endothelial cell responses to laminar flow: changes in gene expression, protein, and glycocalyx
Author(s) -
Meledeo Michael Adam,
Bynum James A,
Sondeen Jill L,
Bowman Phillip D
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1129.10
Subject(s) - umbilical vein , blot , glycocalyx , microbiology and biotechnology , cd31 , flow cytometry , angiogenesis , endothelial stem cell , gene expression , confocal microscopy , laminar flow , biology , endothelium , chemistry , gene , biochemistry , in vitro , genetics , physics , thermodynamics
Previous studies have shown that endothelial cells (ECs) respond to laminar flow and behave more like ECs in vivo. The purpose of this study was to determine the effects of laminar flow on endothelial cells from large compared to small vessels with gene expression analysis, western blotting, and confocal microscopy. Human umbilical vein endothelial cells and dermal microvascular endothelial were grown in Ibidi μ‐VI chamber slides and exposed to 10 or 0.1 dynes/cm 2 or static conditions for 24 h. RNA from replicate chambers were isolated, labeled and hybridized to Agilent human whole genome microarrays. Western blots from the same samples were prepared and probed for Syndican‐2, CD31, CD44, CD31 and heparan sulfate. Fixed specimens were prepared for confocal microscopy. Microarray analysis showed that several hundred genes were either up‐ or down‐regulated when ECs were subjected to laminar flow of 10 dynes/cm 2 compared to static or low flow. These included gene networks associated with cell morphology, cellular movement, angiogenesis, and inflammation. Changes in cell morphology and rearrangement of glycocalyx elements were observed following flow treatment in both ECs. Funding provided by USAMRMC.