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Systemic Leptin Alters Response of Nucleus Tractus Solitarius Neurons That Innervate Rostral Ventrolateral Medulla to Peripheral Chemoreceptors
Author(s) -
Ciriello John,
Moreau Jason M.,
Messenger Scott A.,
Iqbal Waseem,
Migchels Megan J.
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1128.7
Subject(s) - rostral ventrolateral medulla , peripheral , chemoreceptor , medulla , peripheral chemoreceptors , central chemoreceptors , leptin , neuroscience , solitary nucleus , medulla oblongata , nucleus , medicine , chemistry , biology , central nervous system , receptor , obesity
Experiments were done in anesthetized rats to investigate the effects of systemic injection of leptin on the response of neurons within nucleus tractus solitarius (NTS) that innervate the rostral ventrolateral medulla (RVLM) during activation of carotid chemoreceptors. The NTS region containing neurons expressing the leptin receptor Ob‐Rb was explored for single units antidromically activated by stimulation of pressor sites in RVLM. Antidromically activated units were tested for their response to injection of leptin (50 ng/0.1 ml) and KCN (80 μg/0.1 ml). Of 64 units tested in NTS, 28 units were antidromically activated by RVLM and of these units, 17 (~61%) were excited by intracarotid injections of KCN. Additionally, 11 (~65%) of these KCN responsive units were also excited by leptin injections. Furthermore, the response of these single units to KCN was potentiated (83%) immediately following the leptin injection. Control intracarotid injection of saline (vehicle for all injections) neither altered the activity of the NTS neurons nor the response of NTS units to KCN. Taken together, these data suggest that increases in circulatory levels of leptin can alter the sympathoexcitatory responses to peripheral chemoreceptor reflex activation by modulating the activity of neurons within the NTS. Supported by HSFO