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Biodistribution of mesenchymal stem cells in the treatment of experimental caustic esophageal injury
Author(s) -
Caliskan Bahadir,
Kantarcioglu Murat,
Guven Ahmet,
Karacali Ozgur,
Ural Ali Ugur,
Surer Ilhami
Publication year - 2012
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.26.1_supplement.1121.6
Subject(s) - mesenchymal stem cell , biodistribution , homing (biology) , medicine , lung , bone marrow , nuclear medicine , pathology , chemistry , biology , biochemistry , in vitro , ecology
Aim In our study, we investigate experimentally biodistribution of mesenchymally stem cells (MSCs) after injection in early period of caustic esophageal injury especially due to alkaline agents or toher various chemical substances ingestions for accidentally drink. Method and Material In the study, 23 Spraque Dawley rats in total of 3 groups were used. Our study groups designed as Positron Emission Tomograhy (PET) group (n=12), control group (n=7) and MSCs/bone marrow production group (n=4). Caustic esophageal injury created by injection of %20 NaOH solution. This method is described by Gehanno and Guedon. After 24 hours caustic esophageal injury, Fluorodeoxyglucose (FDG) marked MSCs dose at 1 million cells/rat injected into rats tail vein. One hour and 4 hours after injection PET imaging was performed. Results At first hour after FDG injection, FDG uptake was observed in our rats brain, muscle and bladder tissue and FDG marked MSCs settled in the lung. On the other hand at 4 hours after injection FDG marked MSCs settled at lung and also migrated to injured esophageal tissue. When screening with barium esophageal gavage, damaged esophageal tissue in the migration of MSCs marked with FDG was confirmed. Conclusion We demonstrated that after 4 hours MSCs injection via peripheral vein for treatment of esophageal injury due to caustic chemical substances, MSCs settled at lung and small amount of MSCs migrated to injured area (homing). Our findings seems to support of homing feature of MSCs in acute injured tissue when infused via peripheral vein.